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Autotetraploid Pacific oysters (Crassostrea gigas) obtained using normal diploid eggs: induction and impact on cytogenetic stability ArchiMer
Benabdelmouna, Abdellah; Ledu, Christophe.
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the...
Tipo: Text Palavras-chave: Oyster; Tetraploid induction; Mosaicism; Aneuploidy; Cytogenetic stability.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00278/38924/37464.pdf
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